Workflows


Microtubule end tracking

graemeball's picture
Workflow Author: 

Graeme Ball

Dependencies: 
Workflow Language: 
Dimension: 
2D time series
Comment / Instructions: 

Microtubule end tracking in live cell fluorescent images (e.g. to characterise organisation of the cytoskeleton) involves overcoming the following challenges, which can be tackled by a series of filtering steps [1]:

  • illumination flicker & photobleaching: suppress by normalising intensities, e.g. using Image->Adjust->Bleach Correction in Fiji/ImageJ
  • uneven illumination: Fourier bandpass filtering (e.g. Process->FFT->Bandpass Filter) preserves features within a selected size range
  • high background / poor contrast: foreground filter, e.g. Temporal Median filter
  • tracking: e.g. TrackMate in Fiji/ImageJ (segmentation using DoG detector)

References

Rating: 
3
Average: 3 (1 vote)