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Tracking of focal adhesions in 2D time lapse movies

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Workflow Author: 

T. WÜRFLINGER, I. GAMPER, T. AACH and A.S. SECHI

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Tracking of focal adhesions includes a number of challenges:

1. Detection of focal adhesion regions in areas of highly variable backgroud
2. Sepearation of "clumped" adhesions in different objects.
3. Dynamics: Focal adhesions dynamically, grow, shrink, change their shape, they can fuse with neighboring adhesions or one adhesion can be split into multiple children.

The autors describe how to detect focal adhesion objects and how to track them over time[1]. Interestingly, tracking results are fed back to segmentation to improve separation of clumped adhesions.

The authors implemented the workflow in Matlab, but do not provide a ready to use script.

References

  1. [2604] Würflinger T, Gamper I, Aach T, Sechi AS
    2011.  Automated segmentation and tracking for large-scale analysis of focal adhesion dynamics.. J Microsc. 241(1):37-53.
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