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3D estimation of synaptic vesicle distribution in serial section TEM (ssTEM)

tstoeter's picture
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A Great Guy

Comment / Instructions: 

An estimate of the shortest distance of vesicles to synaptic cleft is computed in 3D for serial section TEM. Unfortunately the the authors do not provide an implementation.

Method:
1. Bias correction for inhomogene lighting
2. Image registration of TEM sections / stacks
3. Detection of vesicles & synaptic cleft (semi-automatic)
4. Compute distances in 3D

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