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Lymphatic Vessel Density calculation

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Cédric Balsat, Nicolas Signolle

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2D
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This article [1] present a method to compute Lymphatic Vessel Density on an image of the whole slide.

Vessels are obtained with a Maximum Entropy Thresholding applied on the excess Red channel (2 times the red values minus blue+green value).
Stroma tissue is obtained with a Moment Preserving Thresholding on the blue channel.

References

  1. [2610] Balsat C, Blacher S, Signolle N, Beliard A, Munaut C, Goffin F, Noel A, Foidart JM, Kridelka F
    2011.  Whole slide quantification of stromal lymphatic vessel distribution and peritumoral lymphatic vessel density in early invasive cervical cancer: a method description.. ISRN Obstet Gynecol. 2011:354861.
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neubias's picture

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does it work in 3d?