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Sensitized Emission FRET calculation on pictures

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Workflow Author: 

Laurent Gelman

Dependencies: 
Workflow Language: 
Dimension: 
2D
Input data type: 
Stacks with 2 channels (controls for bleed-through calculation) or 3 channels (FRET calculation)
Output data type: 
One Image with FRET values and One image with normalized FRET values
Comment / Instructions: 

The ImageJ plugin, called PixFRET, allows a simple and rapid determination of channel bleed-through parameters and the display of normalized FRET images. see [1]

References

  1. [2521] Feige JN, Sage D, Wahli W, Desvergne B, Gelman L
    2005.  PixFRET, an ImageJ plug-in for FRET calculation that can accommodate variations in spectral bleed-throughs.. Microsc Res Tech. 68(1):51-8.
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