Sample Data


alkaline comet assay image

Perrine's picture
Brief description: 

from [1] L6 cells were combined with molten LM Agarose (Trevigen) (at 37 °C) at a ratio of 1:10 (v/v), and immediately spread onto CometSlide (Trevigen). After 10 min of gelling at 4 °C, the cells were lysed with lysis solution (Trevigen, USA) at 4 °C for 1 h. Upon three washes in distilled water for 5 min each, alkaline comet assay was performed on the slides plated with L6 cells. In case of alkaline comet assay, the slides were placed in a horizontal electrophoresis tank containing electrophoresis solution (0.3 M NaOH (Merck, Germany), and 1 mM EDTA (Bio Rad, USA), pH>13) and allowed to denature for 30 min. Electrophoresis was run for 25 min (1 V/cm).The slides were rinsed with 0.4 M Tris, pH 7.5 neutralization buffer for 3× 5 min. The slides were then washed in distilled water and immersed in 70% ethanol for 5 min and dried at 40 °C for 10–15 min, and then stained with SYBR green 1 dye (Molecular probes, Invitrogen, USA). Comet images were captured using a Zeiss Axioplan fluorescent microscope.


References

Biological object: 
Cultured cell
Task / Annotation description: 
Licensing: 
Already has a license
Additional license: 
Creative Commons Attribution License (see associated paper copyright)
Author: 
Gyori and Venkatachalam
Scale: 
Single cell
Size (MB): 
0
Dimensions of the data: 
X:251 Y:77 C:1
Thumbnail: