Workflows


comet assay analysis

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Workflow Author: 

Gyori et al

Dependencies: 
Workflow Language: 
Dimension: 
2D
Input data type: 
tiff, 1 channel
Output data type: 
image with outlines, speadsheet
Applicable to (Sample Data):: 
Comment / Instructions: 

This workflow will batch process a directory of images: - comets should be horizontally oriented, tails to the right (additional pre-step if needed top reach this requirement: Rotate images, Using ImageJ/Transform Image or TransformJ plugin for exemple) - then included in the plugin: ->Uneven background correction -> automatiic detection of comet shapes with outliers detection -> automatic detection of head of comets (brightest region or profile analysis) -> statistics aboit tails, heads and Olive moments - Manual correction is also available Can be run from Micro-Manager for live analysis See [1] for associated paper.


References

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