Sample Data


BBBC002v1

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Brief description: 

Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and mad2) has a different gene knocked down by RNAi. The sample preparation is described in more detail by Carpenter et al. (Genome Biology, 2006)

There are 10 fields of view of each sample, for a total of 50 fields of view. The images were acquired on a Zeiss Axiovert 200M microscope. The images provided here are a single channel, DNA. The image size is 512 x 512 pixels. The images are provided as 8-bit TIFF files.

This image set belongs to the Broad Bioimage Benchmark Collection (BBBC). The BBBC resource is described in the following publication: Ljosa V, Sokolnicki KL, Carpenter AE (2012). Anno tated high-throughput microscopy image sets for validation. Nature Methods 9(7):637 / doi. PMID: 22743765 PMCID: PMC3627348. Available at http://dx.doi.org/10.1038/nmeth.2083

Biological object: 
Drosophila melanogaster Kc167 cells
Task / Annotation description: 
Licensing: 
No
Author: 
Anne Carpenter
Scale: 
Many cells
Size (MB): 
4
Dimensions of the data: 
X:512 Y:512 C:1
Comments: 
Recommended citation: "We used the image set BBBC002v1 [Carpenter et al., Genome Biology, 2006] from the Broad Bioimage Benchmark Collection [Ljosa et al., Nature Methods, 2012]."
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