Sample Data


BBBC003v1 MOUSE EMBRYOS

uxisarmi's picture
Brief description: 

Fluorescence microscopy cannot be used to image human embryos to determine embryo viability for in vitro fertilization because the introduction of exogenous fluorescent dyes is considered a toxic procedure. As a result, embryo viability has been measured primarily using differential interference contrast (DIC). A human can readily segment the embryo (and, to some extent, individual cells) in a DIC image, but automatic segmentation remains a challenge due to the cosine-dependent shading inherent in DIC images.

There are 15 images. The images were acquired using a Nikon Eclipse TE200 microscope with a 20x, 0.45 NA objective lens and a 0.52 NA condenser lens, and are provided courtesy of the W.M. Keck 3D Fusion Microscope Facility at Northeastern University. Each image contains 640 x 480 pixels with an approximate size of 0.42 x 0.42 μm.

Biological object: 
Mouse embryos
Task / Annotation description: 
Licensing: 
Already has a license
Additional license: 
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License
Author: 
Pekka Ruusuvuori
Scale: 
Many cells
Size (MB): 
3
Dimensions of the data: 
X:640 Y:480 C:1
Comments: 
Recommended citation "We used image set BBBC003v1 from the Broad Bioimage Benchmark Collection [Ljosa et al., Nature Methods, 2012]."
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