object detection

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SLDC

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Analyze Particles

Package/Library: 
Fiji
ImageJ
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Volocity

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BioImageXD

BioImageXD is a free open source software package for analyzing, processing and visualizing multi-dimensional microscopy images. It's a collaborative project, designed and developed by microscopists, cell biologists and software engineers from the Universities of Jyväskylä and Turku in Finland, Max Planck Institute CBG in Dresden, Germany and collaborators worldwide. BioImageXD was published in the July 2012 issue of Nature Methods.

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u-track

u-track is a multiple-particle tracking Matlab software that is designed to (1) track dense particle fields, (2) close gaps in particle trajectories resulting from detection failure, and (3) capture particle merging and splitting events resulting from occlusion or genuine aggregation and dissociation events. Its core is based on formulating correspondence problems as linear assignment problems and searching for a globally optimal solution.

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Matlab Computer Vision System Toolbox

The Matlab Computer Vision System Toolbox extends the Matlab core functionality with general purpose image processing functions for feature detection & extraction, object detection & tracking and motion estimation.

Strengths:
- Most functions extend to nD
- optimized functions (muti-threaded for some)
- Matlab community (Matlab central)
- relatively low entry-threshold for functionality
- Tutorials & Webinars

Limitations:
- no embedded visualization of nD Microscopy data

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Imaris

Imaris is a software for data visualization, analysis, segmentation and interpretation of 3D and 4D microscopy images. It performs interactive volume rendering that lets users freely navigate even very large datasets (hundreds of GB). It performs both manual and automated detection and tracking of biological “objects” such as cells, nuclei, vesicles, neurons, and many more. ImarisSpots for example is a tool to detect “spherical objects” and track them in time series. Besides the automated detection it gives the user the ability to manually delete and place new spots in 3D space.

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CellProfiler

CellProfiler is designed to enable biologists without training in computer vision or programming to quantitatively measure cell or whole-organism phenotypes from thousands of images automatically. The researcher creates an analysis pipeline from modules that find cells and cell compartments, measure features of those cells to form a rich, quantitative dataset that characterizes the imaged site in all of its heterogeneity.

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ilastik - Object Classification

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